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The poly-ε-lysine and hyaluronic acid (PEL-10/HA144-1) coating is sprayed on titanium (Ti) and ultra-high-molecular-weight-polyethylene (UHMWPE). Coating characterization, antibacterial properties, and biocompatibility were assessed in parallel. All bacteria are American Type Culture Collection (ATCC) strains. Mouse fibroblasts (L929, ATCC cell line CCL 1, NCTC clone 929) and human osteoblast-like cells (Saos-2, CLS, CLS 300331, epithelial-like) were used for biocompatibility assessment.

Journal: PLOS One

Article Title: In vitro assessment of antibacterial and biocompatibility properties of a poly-ε-lysine and hyaluronic acid contact-killing coating to prevent prosthetic joint infection

doi: 10.1371/journal.pone.0340632

Figure Lengend Snippet: The poly-ε-lysine and hyaluronic acid (PEL-10/HA144-1) coating is sprayed on titanium (Ti) and ultra-high-molecular-weight-polyethylene (UHMWPE). Coating characterization, antibacterial properties, and biocompatibility were assessed in parallel. All bacteria are American Type Culture Collection (ATCC) strains. Mouse fibroblasts (L929, ATCC cell line CCL 1, NCTC clone 929) and human osteoblast-like cells (Saos-2, CLS, CLS 300331, epithelial-like) were used for biocompatibility assessment.

Article Snippet: L929 mouse fibroblasts (ATCC cell line CCL 1, NCTC clone 929) were cultured in supplemented Dulbecco’s modified Eagle’s medium (DMEM/F12 low glucose & GlutaMAX, Invitrogen, Carlsbad, CA, USA) supplemented with 10% Fetal Calf Serum (FCS) and 1% antibiotic antimycotic solution (anti-anti; Gibco, USA).

Techniques: High Molecular Weight, Bacteria

As found by the WST-1 assay for the L929 cells (A) and Saos-2 cells (C) , and corresponding cytotoxicity as found by the LDH assay (B for L929 and D for Saos-2 cells). Green bars represent Titanium samples, and green/black striped bars represent UHMWPE samples. Each bar represents the average ± SD of three experiments, each including three samples. Significant differences as found by the Kruskal-Wallis multiple comparison test are displayed in (A) and (C) for untreated cells versus all other samples, and in all graphs for the uncoated sample extract versus all percentages of the coated samples. *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: PLOS One

Article Title: In vitro assessment of antibacterial and biocompatibility properties of a poly-ε-lysine and hyaluronic acid contact-killing coating to prevent prosthetic joint infection

doi: 10.1371/journal.pone.0340632

Figure Lengend Snippet: As found by the WST-1 assay for the L929 cells (A) and Saos-2 cells (C) , and corresponding cytotoxicity as found by the LDH assay (B for L929 and D for Saos-2 cells). Green bars represent Titanium samples, and green/black striped bars represent UHMWPE samples. Each bar represents the average ± SD of three experiments, each including three samples. Significant differences as found by the Kruskal-Wallis multiple comparison test are displayed in (A) and (C) for untreated cells versus all other samples, and in all graphs for the uncoated sample extract versus all percentages of the coated samples. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: L929 mouse fibroblasts (ATCC cell line CCL 1, NCTC clone 929) were cultured in supplemented Dulbecco’s modified Eagle’s medium (DMEM/F12 low glucose & GlutaMAX, Invitrogen, Carlsbad, CA, USA) supplemented with 10% Fetal Calf Serum (FCS) and 1% antibiotic antimycotic solution (anti-anti; Gibco, USA).

Techniques: WST-1 Assay, Lactate Dehydrogenase Assay, Comparison